ZFIN Image: Veroni et al., 2024, Figure 5

Image

Figure Caption

Figure 5

EDA induction of AHR target genes in the SH-SY5Y neuroblastoma cell line. (a) SH-SY5Y cells were incubated with 30 µM EDA, 100 µM EDA, 1 µM INDI or DMSO alone for 14 h. Total RNA was extracted and the expression of AHRR and CYP1A1 transcripts was evaluated using qPCR. Data are expressed as 2^−ΔCt relative to the housekeeping gene GAPDH. (b–d). SH-SY5Y cells were treated with 100 µM EDA, 1 µM INDI or DMSO alone for 24 h and CYP1A1 (b), AHRR (c) and NRF2 (d) protein expression was investigated by Western blot. Data are expressed as the ratio between AHR and the GAPDH reference. Bars represent the mean ± SEM of 3 experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 using unpaired Student’s t-test. The image was edited using BioRender.com (https://www.biorender.com/ accessed on 3 April 2024).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biomolecules