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Fig. 3 tPA-functionalized µwheels are necessary for recanalization. The PCV of 3 dpf larvae was injured, producing completely occlusive thrombi, followed by no infusion (n = 18), the infusion of microparticles functionalized with ~8.4 pg of tPA (n = 16), unfunctionalized µwheels (n = 15), or tPA-functionalized µwheels carrying ~8.4 pg of tPA (n = 15). The field was applied in rolling mode either until recanalization was observed or a maximum of 30 min. Beyond that point, the coils became too hot and damaged the larvae. Larvae infused with tPA-functionalized µwheels recanalized within 11.8 ± 5.4 min while those infused with non-functionalized µwheels, tPA-functionalized particles without the application of a magnetic field, and uninfused larvae continued to be occluded after 30 min (P < 0.0001 by Mantel-Cox log-rank testing). Bars represent median recanalization time.