ZFIN Image: Manzoli et al., 2024, Fig. 5

Image

Figure Caption

Fig. 5 Proteasome inhibition in wild-type primary neurons results in Dcc upregulation.

A Western blot analysis on 2 DIV wild-type and ids mutant fish-derived primary neurons showed significant upregulation of polyubiquitinated proteins and P62, as depicted by the bar graphs on the right. On the other hand, no differences were found for Lc3-II (n = 6 biological replicates). B Wild-type fish-derived primary neurons were treated with MG-132 (proteasome inhibitor) for 1, 6, or 16 hours and then analysed at 2 DIV. On the left, representative western blots for Dcc, ubiquitin and P62. After 16 hours of treatment, Dcc levels were significantly increased when compared to DMSO (n = 3 independent experiments). Data are expressed as the mean ± SD (*p < 0.05; **p < 0.005; ***p < 0.001; t-test).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Death Dis.