Fig. 3

Fig. 3 ids mutant fish-derived primary neurons exhibit Dcc upregulation with enrichment of Dcc-positive puncta.

A Significant Dcc upregulation in ids mutant fish-derived primary neurons was detectable since 6 HIV (at least n = 4 biological replicates) and maintained at 2 DIV (n = 7 biological replicates), as depicted by western blots and related bar graphs. IDS recombinant enzyme (Elaprase) treatment of ids mutant-derived cells restores Dcc levels to those of wild-type cells (n = 7 biological replicates). B Representative Dcc immunofluorescence on wild-type and ids mutant fish-derived primary cells at 2 DIV. Acetylated tubulin (green) was used to trace neuronal processes. The bar graph on the right displays the quantification of Dcc-positive aggregates per cell. The magnification highlights Dcc-positive spots (n = 4 independent experiments). Scale bar: 50 μm. Data are expressed as the mean ± SD (**p < 0.005; t-test).