Figure 6

Figure 6

Cavin1a knockout zebrafish embryos show decreased abundance of caveolar proteins in the sarcolemma

(A) EM images of slices of muscle tissue in wild-type (top) and Δcavin1a zebrafish embryos (bottom). White arrows point to caveolar structures. Scale bar, 100 nm.

(B) Number of caveolae in muscle and notochord cells of wild-type and Δcavin1a zebrafish embryos. Each data point corresponds to the value determined for one muscle cell.

(C) Representative fluorescence images of wild-type and Δcavin1a strains injected with caveolin3:Clover. Scale bar, 5 μm.

(D) Abundance of caveolar proteins tagged with Clover in the Z-lines, relative to the abundance in the sarcolemma. Error bars represent the SD. The significance was tested using the Bonferroni and Holm t test.

(E) Abundance of TinyDysf-Clover in the Z-lines, relative to the abundance in the sarcolemma.