Fig. 10.

Fig. 10.

Inhibition of BMP signalling by heat shocking dnBMPR zebrafish embryos reduces cartilage maturation gene expression and perichondral bone formation. (A-H) Compared with chondrocytes in non-heat-shocked wild-type (A,B) and dnBMPR (C,D) embryos and heat-shocked wild-type (E,F) embryos, heat-shocked dnBMPR (G,H) chondrocytes had decreased expression of the chondrocyte maturation genes ihha and col10a1a at 4 dpf (i.e. 3 days after heat shock). These are representative images from at least 12 samples for each group (at least six samples each from two clutches). (I-L″) Compared with control groups (I-K″), perichondral bone appeared to decrease in heat-shocked dnBMPR embryos (L-L″) at 7 dpf (i.e. 6 days after heat shock). (M) Quantitation of 100 embryos (20 embryos each from five clutches) for each experimental group confirmed a significant decrease in perichondral bone in heat-shocked dnBMPR embryos (*P<0.05, one-way ANOVA and paired Student's t-test). Scale bars: 50 μm (A-H); 200 μm (I-L″). A, anterior; AB/AR, Alcian Blue/Alizarin Red; Ch, ceratohyal cartilage; Chb, ceratohyal bone; Hm, hyomandibular bone; Hs, hyosymplectic cartilage; HS, heat-shocked; L, lateral; wt, wild type.