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Fig. 2 Cd-induced vascular endothelial damage was abrogated/exacerbated by Fer-1/Erastin in zebrafish embryos. (A) Zebrafish embryos were pretreated with ferrostatin-1 (Fer-1, 1 μM) or erastin (5 μM) and co-treated with 100 μM CdCl2. ROS were observed by fluorescence microscopy, and the relative level of ROS was counted (B). The contents of LPO and MDA (C) per mg weight were examined, and the fold changes in GSH, GSSG (D) and GSH/GSSG (E) were observed under Cd stress. (F) kdrl:mCherry × fli1a:nGFP+ ECs in the tail were observed. The number of ECs (G) and relative area of CVP (H) with various treatments were counted (n = 5). The values are presented as the mean ± SEM in histograms or as median (line), while whiskers mean min to max levels in boxplots. *P < 0.05, **P < 0.01 and ***P < 0.001 (unpaired t test or one-way ANOVA followed by multiple comparisons).