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Fig. 3 Initiator and executioner caspase cleavage is significantly reduced when septins are depleted. (a), (b) HeLa cells were transfected with control or SEPT7 siRNA for 72 hr. Cells were then treated with DMSO or (a) 1 μM STS or (b) 500 μM ETP for 10 hr. Whole-cell lysates were immunoblotted against caspase-3, cleaved caspase-3, caspase-7, caspase-9, and GAPDH (used as loading control). Data are representative of three independent experiments. (c) SEPT2 is required for caspase activation. HeLa cells were transfected with control or SEPT2 siRNA for 72 hr. Cells were then treated with DMSO or 1 μM STS for 10 hr. Whole-cell lysates were immunoblotted with antibodies to caspase-7, caspase-9, and GAPDH. Data are representative of three independent experiments. (d) HeLa cells were transfected with control or SEPT7 siRNA for 72 hr. Cells were then treated with DMSO or 1 μM STS for 10 hr. Whole-cell lysates were immunoblotted with antibodies to caspase-4, caspase-7, caspase-9, and GAPDH. Data are representative of three independent experiments