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Fig. 7 Model of Vegfc function in determining HE fate decisions in the DA. (A) Schematic of HSPC development in control embryos. Emergence of nascent HSPCs from the DA peaks at 48 hpf. In late stage EHT (56 hpf and later) there is a decrease in HSPC emergence and the appearance of few crawling cells. HSPCs then colonize the CHT, where there are also myeloid cells present. (B) Schematic of HSPC development in vegfc loss-of-function embryos. There are significantly fewer HSPCs during the peak of emergence from the DA. In late stage EHT there is a change in cell behavior and many crawling cells emerge from the DA. In the CHT there are decreased HSPCs and an increased number of myeloid cells