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Fig. 4 blf and the drl cluster regulate macrophage versus neutrophil fate choice during primitive myelopoiesis. (A) Left: RNA in situ hybridization of mpx, lyz, mpeg1, mfap4.1 and coro1a expression at 23 hpf. Right: The number of myeloid cells in different genotypes for each marker. Scale bars: 100 μm. (B) Left: Merged confocal images of the uncaged fluorescein and FISH signals in the trunk at 72 hpf. Scale bars: 50 μm. White arrowheads indicate the overlapping of fluorescein and FISH staining. Right: Quantification of fluorescein-labeled myeloid cells adopting the neutrophil or macrophage fate in control and blf−/−; drl 4KO embryos. Nmpx+, number of mpx-expressing cells; Nmpeg1+, number of mpeg1-expressing cells; NFlu+, number of fluorescein-labeled cells. Quantification was carried out on a 150 μm×275 μm field. Data were collected for 12-16 embryos per experiment group. n.s, not significant, *P<0.05, **P<0.01, ***P<0.001 (t-test).