Fig. 3

Fig. 3 Pulsed-SBS imaging of zebrafish larvae and young adult C. elegans.

a, Illustration of a zebrafish larva, indicating the imaging region (dashed red box). b, Brightfield image of the notochord region of a zebrafish larva at 3 dpf stage. c, Brillouin shift image of the x–y plane of the region marked by a red dashed box in b. d, Cross-sectional Brillouin shift image in the y–z plane along the yellow dashed line in b. Scale bars in b–e are 5 µm. In c and d, the pixel steps in x and y directions are both 0.2 µm and the pixel time is 40 ms. e, Multi-peak metric map of the region marked by a red dashed box in b. f, A representative Brillouin spectrum in the high-shift region, indicative of ECM, marked by a cross in c and e. The raw spectrum and its triple-peak fitting are shown (L1–3). Note that an arbitrary offset was added to L1–3 for visualization. g, A representative Brillouin spectrum in the blood vessel region marked by a plus sign in c and e. The raw spectrum and its double-peak fitting (L1 and L2) are shown. Note that the offset was added to L1 and L2 for visualization. h, Schematics of a young adult C. elegans. The head and gonad regions imaged are marked by dashed red boxes. i,j, Brillouin shift images of the head region under 27 mW power (i) and 250 mW power (j). Note that slight movements of the nematode during high-power imaging blur out the contrast (c.f. i). In i and j, the pixel steps in x and y directions are both 0.5 µm and the pixel time is 20 ms. k,l, Brillouin shift image (k) and coregistered brightfield image (l) of the gonad region marked by a dashed red box in h, showing oocyte nuclei from different meiotic stages (arrows). In k, the pixel steps in x and y directions are both 0.25 µm and the pixel time is 20 ms. Scale bars in i–l are 10 µm.

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