ZFIN Image: Sun et al., 2023, Figure 2

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Figure 2

Proteomic analysis of isolated LDs from zebrafish liver. A Proteins were separated by SDS-PAGE after extracting from LD, TM, Cyto, and PNS fractions, and then stained by colloidal blue. Red arrowheads marked unique protein bands of LDs. B Proteins of LDs were precipitated by acetone and then subjected to nano-LC-ESI-LTQ Orbitrap XL MS/MS analysis. The Venn diagram showed the overlap of two independent mass spectrum results. C The 259 proteins of LDs identified in two samples were categorized into 11 groups: perilipin family, lipid metabolism, mitochondria, endoplasmic reticulum, cytoskeleton, chaperone, cell signal, membrane trafficking, protein translation and modification degradation, other protein and unknown function protein. D Proteins of LDs of two independent mass spectrum results were categorized into previously reported proteins and newly identified proteins in this study

Acknowledgments

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