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Fig. 2 spic deficiency results in the depletion of metaphocytes (A) Schematic outline of the generation of spic mutants by CRISPR-Cas9. (B) Representative images of the epidermis and gill of Tg(grn2:EGFP;mpeg1:DsRedx);spicΔ136 mutants and siblings. EGFP+DsRedx+ cells are metaphocytes and EGFP−DsRedx+ cells are macrophages. Scale bar, 50 μm. (C) qPCR detection of the relative expression level of metaphocyte marker genes in the epidermis and gill of spicΔ136 mutants and siblings. eef1a1l1 was utilized as the internal control. Values are represented as mean ± SD (n = 4). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. epi, epidermis; sib, sibling; mut, mutant. See also Figure S2.