PUBLICATION

Metaphocytes are IL-22BP-producing cells regulated by ETS transcription factor Spic and essential for zebrafish barrier immunity

Authors
Zhao, C., Li, Y., Tang, J., Zhou, Q., Lin, X., Wen, Z.
ID
ZDB-PUB-230507-45
Date
2023
Source
Cell Reports   42: 112483112483 (Journal)
Registered Authors
Wen, Zilong, Zhou, Qiuxia
Keywords
CP: Immunology
Datasets
GEO:GSE228732, GEO:GSE228737, GEO:GSE228748
MeSH Terms
  • Animals
  • Cell Differentiation
  • DNA-Binding Proteins
  • Dendritic Cells*
  • Mammals
  • Mice
  • Receptors, Interleukin/metabolism
  • Zebrafish*
PubMed
37148242 Full text @ Cell Rep.
Abstract
Metaphocytes are tissue-resident macrophage (TRM)/dendritic cell (DC)-like cells of non-hematopoietic origin in zebrafish barrier tissues. One remarkable property of metaphocytes is their ability to capture soluble antigens from the external environment via transepithelial protrusions, a unique function manifested by specialized subpopulations of the TRMs/DCs in mammal barrier tissues. Yet, how metaphocytes acquire myeloid-like cell properties from non-hematopoietic precursors and how they regulate barrier immunity remains unknown. Here, we show that metaphocytes are in situ generated from local progenitors guided by the ETS transcription factor Spic, the deficiency of which results in the absence of metaphocytes. We further document that metaphocytes are the major IL-22BP-producing cells, and the depletion of metaphocytes causes dysregulated barrier immunity that resembles the phenotype of IL-22BP-deficient mice. These findings reveal the ontogeny, development, and function of metaphocytes in zebrafish, which facilitates our understanding of the nature and function of the mammalian TRM/DC counterparts.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping