Figure 5.

Figure 5.

ROCK inhibition does not impair myogenesis during regeneration. Control injured pax7a:egfp larvae (injury location marked by asterisk) or those treated with 10 µM Y-27632 were exposed to BrdU for 24 h (a, enlarged area in b). Quantification of GFP+ cells (c) and BrdU + cells (d) in control and Y-27632 treated pax7a:egfp animals at 24 hpi. The number of GFP+ cells and number of nuclei per GFP+ cells was assessed at 48 hpi in control and Y-27632 treated animals (e, enlarged area in f). Comparison of the relative number of GFP+ cells in regenerating muscle of control animals compared to those treated with Y-27632 (g). Relative nuclear number per newly regenerating GFP + fibre is shown for control and Y-27632 treated animals and represented as classes with 1 nuclei (1 > 1), between 2 to 3 (2 > 3), between 4 to 5 (4 > 5) or more than 6 nuclei per GFP + myofibre (h). Significance was calculated using t-test with Bonferroni correction (c,d), by 2-way ANOVA with Tukey's post-hoc correction (g) or by a Chi-squared test (h) with n = 3 animals per condition. Scale bars 100 µm (a,e), 10 µm (b,f).