Fig. 2.

Fig. 2. Ablating EG neutrophils reduces survival to subsequent infection.

(A) Schematic illustrating strategy to ablate SS and EG neutrophils. Tg(lyz:YFP-NTR2.0;mpeg1:nfsB-mCherry) SS larvae and those demonstrating EG were treated with dimethyl sulfoxide (DMSO; control) or 0.2 mM mtz. At 6 hours posttreatment (hpt), neutrophil-ablated larvae were infected with Sal-GFP and treated continuously with 0.1 mM mtz. (B) Flow quantification of YFP-expressing neutrophils from whole SS and EG Tg(lyz:YFP-NTR2.0;mpeg1:nfsB-mCherry) larvae, 6 hours following mtz treatment, compared to DMSO-treated controls (n = 20 larvae per sample, five experimental replicates). (C) Flow quantification of mCherry-expressing macrophages from whole SS and EG Tg(lyz:YFP-NTR2.0;mpeg1:nfsB-mCherry) larvae, 6 hours following mtz treatment, compared to DMSO-treated controls (n = 20 larvae per sample, five experimental replicates). (D) Kaplan-Meier graph showing survival of DMSO- and mtz-treated SS and EG Tg(lyz:YFP-NTR2.0;mpeg1:nfsB-mCherry) larvae over 5 dpsi with Sal-GFP at 4 dpf. Error bars, mean ± SD; ****P < 0.0001; one-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test (B and C) and Gehan-Breslow-Wilcoxon test (D).