Fig. 1

Knockout of miR-184 in zebrafish. (A) The gRNA was designed to target the identical seed sequence (marked in red) in both dre-mir-184-1 and dre-mir-184-2. (B) The mature miR-184 sequence is extremely conserved among human, mouse, chicken, frog, fish, and fruit fly. (C and D) The deletions of dre-mir-184-1 and dre-mir-184-2 were identified by direct sequencing in miR-184 knockout zebrafish line. (E and F) The deletions of dre-mir-184-1 and dre-mir-184-2 altered the stem-loop secondary structure of zebrafish miR-184 precursors. (G) The levels of mature miR-184 were detected in wild-type (WT) and miR-184 knockout (miR-184^−/−) zebrafish. Student t-test was used for statistical analysis, n = 3, ***p < .001.