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Fig. 5
Exportin 1 (XPO1) inhibitors treatment does not induce DNA double-strand breaks in zebrafish haematopoietic stem and progenitor cells (HSPCs). (A) Immunolabelling was performed on 2.5 days post fertilisation embryos (treated with either dimethylsulfoxide (DMSO), 100 nM selinexor, 250 nM eltanexor or 200 nM topotecan) with the indicated genotypes in the Tg(c-myb:EGFP) background to identify HSPCs (anti-EGFP antibody; green) and DNA double-strand breaks (anti-γH2AX antibody; red) in the caudal haematopoietic tissue (CHT). White arrows indicate groups of HSPCs with DNA double-strand breaks. Magnified views of the regions in the dashed boxes are shown on the right. Scale bar: 25 μm. (B) Quantification of EGFP+; γH2AX+ cells as described in panel A.
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Full text @ Br. J. Haematol.