Fig. 4

Exportin 1 (XPO1) inhibitors treatment induces apoptosis in tet2-mutant zebrafish HSPCs. (A) TUNEL and immunolabelling were performed on 2.5 days post fertilisation (dpf) embryos (treated with either dimethylsulfoxide [DMSO]; 100 nM selinexor, or 250 nM eltanexor) with the indicated genotypes in the Tg(c-myb:EGFP) background to identify haematopoietic stem and progenitor cells (HSPCs) anti- enhanced EGFP antibody, green) and apoptotic cells (TMR-red, red) in the caudal haematopoietic tissue (CHT) region. Fluorescence was visualized using confocal microscopy. White arrows indicate HSPCs undergoing apoptosis. (B) Quantification of EGFP+; TUNEL+ cells as described in panel A.