FIGURE 7

FIGURE 7

Hydroxysafflor yellow A (HSYA) partially repairs hematopoietic defects in 5‐FU‐treated mice: (A) flow cytometry in different treatment groups to detect the proportion of hematopoietic stem cells and the distribution of erythrocytes at different stages on day 10. LSK cells/hematopoietic stem cells (HSCs) (Lin‐Sca‐1⁺c‐kit⁺); proerythroblast/R1 (CD71^highTer119^intermediate); basophilic erythroblast (CD71^highTer119^high); polychromatic erythroblast (CD71^intermediateTer119^high); orthochromatic erythroblast (CD71^lowTer119^high); 5‐FU, 200 mg/kg; HSYA, 100 mg/kg; (B) quantitative result of flow cytometry; (C) flow cytometry to detect the proportion of neutrophils, T cells and B cells on day 10. Neutrophil (CD11b⁺Gr1⁺); T lymphocyte (CD3⁺); B lymphocyte (CD19⁺); (D) quantitative result of (C) for flow cytometry; three mice in each group for flow cytometry; (E) immunohistochemical staining of P53 in mouse thymus tissue. Arrowheads pointed to P53‐stained cells. Scale bar: 20 μm; (F) QPCR results showing the expression of Bax and Caspase3 in different mice treatment groups. *p < 0.05; **p < 0.01.