ZFIN Image: Subramanian et al., 2023, Fig. 3.

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Figure Caption

Fig. 3.

Paralysis regulates expression of scxa, sox9a and col1a1a in the embryonic mc-IMA enthesis. (A-S) Ventral views, anterior to the left, of wild-type (WT) (A,B,F,G,K,L,P,Q) and cacnb1^−/− (C,D,H,I,M,N,R,S) embryos showing expression of scxa (magenta), sox9a (green) and col1a1a (cyan) genes at the mc-IMA tendon enthesis at 48 hpf (A-D), 55 hpf (F-I), 60 hpf (K-N) and 72 hpf (P-S). Plots show quantification of scxa (E), sox9a (J), col1a1a (O) expression and ratio of scxa versus sox9a expression (T) in WT (dotted line) and cacnb1^−/− (solid line) tenocytes for the stages indicated. Error bars show standard deviation. Linear mixed effects model was created and Tukey post-hoc pairwise comparison was performed. *P<0.05, ***P<0.001. Scale bars: 20 μm (A,C,F,H,K,M,P,R); 5 μm (insets, B,D,G,I,L,N,Q,S).

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