FIGURE 5

FIGURE 5

Expression of nrip1a and nr2f2 in adult zebrafish retina. (A–C”’)nrip1a. (D–F”’)nr2f2. (A) Expression of lws1 (magenta) and lws2 (cyan) in a representative whole retina. (A’)nrip1a expression (yellow) in the same preparation, showing pan-retinal expression. (B) 40x image of lws1, lws2, and nrip1a expression in a region of lws1 to lws2 transition. (B’)nrip1a alone, in same region. C-C”’) Resliced orthogonal projection of panel (B). (C) All imaging channels merged. (C’) DAPI and nrip1a. (C”) DAPI and lws1.(C”’) DAPI and lws2.(D) Expression of lws1 (magenta) and lws2 (cyan) in a representative whole retina. D’) nr2f2 expression (yellow), showing slight bias in signal intensity toward dorsal retina. (E) 40x image of lws1, lws2, and nr2f2 expression in a region of lws1 to lws2 transition, at the level of cone inner segments. Scale same as in panel (B). (E’)nr2f2 alone, in the same region, at the level of the deep INL. (F–F”’) Resliced orthogonal projection of panels (E,F) All imaging channels merged. (F’) DAPI and nr2f2. (F”) DAPI and lws1.(F”’) DAPI and lws2. D, dorsal. Sample size = 2.