Fig. 4
Fig. 4. Acridine orange (AO) staining and expression of genes related to apoptosis. (A) Representative fluorescent images of early apoptotic signals detected by AO staining in the DMSO and TPhP treated groups at 96 hpf. (B) Number of apoptotic cells in the head of zebrafish at 96 hpf observed with laser confocal microscopy. The expression of apoptosis-related genes in zebrafish after 96 hpf (C) and 7 dpf (D) exposure to TPhP. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001, compared with the control. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)