Fig. 5

Fig. 5

Fig. 5. : Quantitative and representative results of L. nuda extract treatment-mediated SIV inhibition. (A) The representative results of SIV development following L. nuda extract treatment showed a delay in SIV growth in zebrafish embryos. L. nuda extract (5–100 μg/mL) led to the depletion of SIV sprouting, delayed basket formation, and defects in basket growth. White asterisks and hash indicate normal SIV growth, sprouting, branching, and branching + sprouting. (B) Quantitative results of the number of embryos showing normal and defective SIV development. Control (n = 30) and L. nuda extract treatment (n = 30). (C, D) Quantitative results of SIV growth, sprouting, branching, and branching + sprouting observed in individual embryos in control and L. nuda extract-treated embryos observed at 72 hpf. * p < 0.05, ^##, * * p < 0.001. Scale bar = 100 µm.