ZFIN Image: Sun et al., 2023, Fig. 1

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Figure Caption

Fig. 1 Single-cell RNA-sequencing reveals hapln1a+ epicardial clusters are enriched with angiogenesis factors during coronary vascularization.

a Uniform manifold approximation and projection (UMAP) clustering of tcf21⁺ single-cells from juvenile hearts. b Heatmap of the top 10 markers for epicardial cells from juvenile tcf21:nucEGFP hearts. c Identification of epicardial cell clusters based on gene ontology analysis. d Feature plot of hapln1a expression in epicardial clusters of juvenile hearts. e Heatmap of gene ontology enrichment for tcf21⁺/hapln1a⁺ epicardial cells vs. tcf21⁺/hapln1a^- cells. f Log₂-fold-change vs. abundance of normalized gene expression of tcf21⁺/hapln1a⁺ epicardial cells vs. tcf21⁺/hapln1a^- cells. Each point designates a unique gene; differentially expressed unique genes are in red. Unique genes with higher expression in tcf21⁺/hapln1a⁺ epicardial cells have positive fold-change values, whereas unique genes with higher expression in tcf21⁺/hapln1a^- cells have negative fold-change values.

Acknowledgments

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