ZFIN Image: Rodenas et al., 2023, FIGURE 2

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Figure Caption

FIGURE 2

Hepsin overexpression in Caco-2-HPN cells. (A) The hepsin mRNA levels were deter-mined in Caco-2 and Caco-2-HPN cells by RT-qPCR. Gene expression levels were normalized to b-actin mRNA levels and the data are represented as the mean ± standard error of the mean of technical triplicates. Levels were shown as fold increase relative to the mean of Caco-2 cells. (B) Hepsin protein levels were determined in Caco-2 and Caco-2-HPN cells by electrophoresis and Western blot in lysates of Caco-2 and Caco-2-HPN cells. Vinculin expression was detected as loading control to relativize hepsin levels. Data are represented as the mean ± standard error of the mean of technical triplicates. HPN, Hepsin; Caco-2-HPN, Caco-2 cells overexpressing hepsin; Caco-2, Caco-2 cells with hepsin basal expression; *: p-value < 0.05.

Acknowledgments

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