FIGURE 4

FIGURE 4

pSTAT3, pAKT and pERK1/2 expression in Caco-2 and Caco-2-HPN cells. Expression of pERK1/2 (A), pSTAT3 (B) and pAKT (C) determined by electrophoresis and Western blot in lysates of Caco-2 and Caco-2-HPN cells in triplicates. Levels were determined by densitometry and represented as relative protein expression to vinculin. Graphs represent the mean ± standard error of the mean of the triplicates. (D) Electrophoresis and Western blot of pSTAT3, pAKT and pERK1/2 in lysates of Caco-2 and Caco-2-HPN cells in triplicates. Vinculin expression was detected as loading control. pERK1/2, phospho-extracellular signal-regulated kinases 1 and 2; pSTAT3, phospho-signal transducer and activator of transcription 3; pAKT, phospho-Protein Kinase B; Caco-2-HPN, Caco-2 cells overexpressing hepsin; Caco-2, Caco-2 cells with hepsin basal expression; *: p-value < 0.05; HPN, Hepsin; +, overexpression; -, basal expression.