ZFIN Image: Shi et al., 2023, Figure 3

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Figure Caption

Figure 3

Generation of foxO4^−/− zebrafish via CRISPR/Cas9 technology. (A) The DNA sequence and predicated protein product difference in foxO4 between the mutants and WT. sgRNA sequence is marked with a black box. The green letters in MT represent the mutated DNA sequence. (B) Verification of zebrafish foxO4 disruption by agarose gel electrophoresis. (C) foxO4 mRNA expression levels in foxO4^−/− and WT zebrafish embryos at 96 hpf were detected by qPCR. ** p < 0.01. (D) Observation of the foxO4^−/− and WT adult zebrafish.

Acknowledgments

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