Figure 3

Example of eye malformation. (A) Untreated larva 4 dpf; (B) flattened eye with dents at both the upper and lower side of the eye and (C) eye hypoplasia of treated larvae with POP125×; (D) dose-based prevalence of eye malformation; (E) prevalence of eye malformation in larvae upon treatment with the different POP sub-mixtures. Data is presented as mean percentage of population having eye malformation and standard deviation; ordinary one-way ANOVA and Šidák’s multiple comparison test, n = 100, p ≤ 0.001 (***). Asterisks indicate when significant differences were found compared to control, hash sign (#) when differences were found compared to PFAA. (F) Prevalence of eye malformation present in fish exposed to POP125× relative to untreated individuals. Columns represent the effect of POP125 treatment in larvae previously injected with Mo^p53 (POP125× + injection) or not (POP125×). A Fisher’s exact test revealed the difference with a significance of p = 0.055 (*).