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Fig. 4
(A) Transgene design (not to scale) for macrophage-specific expression of EsxM. The macrophage-specific zebrafish promoter mfap4 drives expression of the mycobacterial protein within zebrafish macrophages. 2A peptide uncouples EsxM protein (98 AA) from tdTomato.
(B) Representative larval zebrafish expressing the mfap4:esxM-p2A-tdTomato transgene. Scale bar, 500 μm.
(C) Increased macrophage motility in the absence of infection for EsxM-expressing transgenic zebrafish lines. Scale bar, 100 μm.
(D) Migration and velocity measured in response to tail fin transection. One-way ANOVA, Dunn’s multiple comparison test. Data representative of three biological replicates.
(E) Number of macrophages recruited to tail wound 90 min post-transection. Macrophages express fluorescent TdTomato, EsxM, or truncated EsxM (Q59X) via a macrophage-specific promoter (mfap4). One-way ANOVA with Dunn’s multiple comparison test. Data representative of three biological replicates.
(F) Maximum velocity of macrophages migrating to tail wound. One-way ANOVA with Dunn’s multiple comparison test. Data representative of three biological replicates. Mean ± SD shown on all graphs. **p < 0.01, ***p < 0.001.
See also Figure S3 and Video S3.
Reprinted from Cell, 185(24), Saelens, J.W., Sweeney, M.I., Viswanathan, G., Xet-Mull, A.M., Jurcic Smith, K.L., Sisk, D.M., Hu, D.D., Cronin, R.M., Hughes, E.J., Brewer, W.J., Coers, J., Champion, M.M., Champion, P.A., Lowe, C.B., Smith, C.M., Lee, S., Stout, J.E., Tobin, D.M., An ancestral mycobacterial effector promotes dissemination of infection, 4507-4525.e18, Copyright (2022) with permission from Elsevier. Full text @ Cell