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Figure 5
Increased apoptosis and decreased proliferation of cranial neural crest cell in vwa1 mutant embryos. (A,B) TUNEL staining (red fluorescence) revealed increased cell apoptosis in the cranial region and dorsal tissues of vwa1 mutant embryos at 24 hpf. SOX10 marked CNCCs. (C,D) PHH3 staining (red fluorescence) showed decreased cell proliferation in the cranial region of vwa1 mutant embryos at 30 hpf. SOX10 marked CNCCs. The pharyngeal arches are marked with dotted white circles. * p < 0.05; *** p < 0.001.
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