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Figure 9
Expression patterns of novel annular ligament associated genes. (A) WISH expression analysis of annular ligament associated genes stmn1a, si:211-251b21.1, hgd, phgdh and cndp1 at 24, 72 and 120 hpf displayed in dorsal and lateral views. (B) 10 µm cryosections of 120 hpf embryo eyes after WISH. (C) Two color FWISH depicting co-expression of stmn1a(green)/scinla(red), si:ch211-252b21.1(green)/scinla(red), hgd(green)/scinla, phgdh(green)/scinla(red) and cndp1(green)/scinla(red) at 120 hpf. DAPI (blue) was used to stain the nuclei. Volume projections in lateral view and single section dorsal views from 3D confocal stacks are displayed. White arrows indicate example regions of co-expression. Scale bar = 100 µm. Individual fluorescence channels are provided in Fig. S11C. (D) Average mean expression measurements over developmental time. ALP Annular ligament progenitor (yellow arrow), CEp Corneal epithelium (blue arrow), CEn Corneal endothelium (green arrow), CSt Corneal stroma (green arrow).