ZFIN Image: Cotellessa et al., 2023, Figure 3

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Figure Caption

Figure 3

Jag1a, jag1b, notch1a, and gnrh3 mRNAs are expressed in the OP of zebrafish embryos at 48 hpf.

(A) WISH on zebrafish embryos at 24 hpf, 32 hpf, and 48 hpf for jag1a and jag1b mRNAs. WISH was replicated 4 times with n = 15 WT embryos. Jag1a and jag1b are both expressed within the OP (arrows). (B) Representative confocal image of the double reporter line tg(12xnre:mCherry × GnRH3:EGFP) showing the expression of GnRH3 (green) and of the NRE (red) in the OBs of zebrafish embryos at 32 hpf. The experiments were replicated 3 times with n = 10 EGFP and mCherry double-positive embryos. (C) WISH on zebrafish embryos at 48 hpf showing notch1a mRNA localization in the OP (arrows). WISH was replicated 4 times with n = 15 WT embryos. Scale bars: 0.5 mm, A and C; 40 μm, B upper panel; 10 μm, B lower panel.

Acknowledgments

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