Fig. 4

Fig. 4 Anisotropic cell shape analysis. (A) Representative confocal image of a WT embryo injected with memGFP and imaged at 24 ss. The dashed yellow lines indicate apical and basal regions of the tissue where digital slices of the dorsal-ventral plane were taken. (B) Three-dimensional diagram of neuroepithelial cells at the MHB. Yellow rectangles represent where digital slices were taken in both the apical and basal regions of the cell. Approximate apical anisotropic shape can be visualized by dotted lines, while basal anisotropic shape can be visualized by narrow blue regions. (C–D) Representative digital slices in the anterior-posterior (x-axis) and dorsal-ventral (z-axis) dimension of apical (C) and basal (D) cellular regions of the MHB. Cells at the MHBC are outlined in yellow. (C′-D′) Representative measurements of cells in x-dimension (blue line) and z-dimension (red line). (E) Diagram of x:z ratios and their relationship to anisotropic cell shape. Scale bars 20 ​μm.