ZFIN Image: Falat et al., 2022, Fig. 2

Image

Figure Caption

Fig. 2 lama1, lamb1a, and lamc1 are all required for proper MHB tissue angle. (A–E) Representative confocal images of memGFP injected embryos imaged at 24 ss, WT (A) and col4a5 (B), lama1 (C), lamb1a (D), lamc1 (E) mutants. (F) Quantification of angle measurements. Boxplots indicate the 25th and 75th percentiles and the median. At least three independent experiments are represented. n = 19 embryos for WT, 6 embryos for col4a5, 9 embryos for lama1 mutants, 6 embryos for lamb1a mutants, and 7 for lamc1 mutants. Significance values ∗∗∗P < 0.005. Yellow arrows along the basal edge of the tissue are representative of angle measurements. White arrowheads indicate the MHBC. M, midbrain; H, hindbrain. Scale bars: 20 μm.

Acknowledgments

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Reprinted from Developmental Biology, 492, Falat, E.J., Voit, G.C., Gutzman, J.H., Laminin-111 mutant studies reveal a hierarchy within laminin-111 genes in their requirement for basal epithelial tissue folding, 172-186, Copyright (2022) with permission from Elsevier. Full text @ Dev. Biol.