Figure 4

DRAM1 knockdown reduces LC3 trafficking to Mm. (A) Representative examples of LC3 (magenta) and LysoTracker (yellow) colocalisation with Mm (cyan) in DRAM1 knockdown (KD1-3) and control (Ctrl) cell lines at 120 min post infection. The arrowheads indicate the colocalisation of LC3, Mm and LysoTracker. Scale bars: 2 μm. (B) Percentage of colocalisation of Mm with LC3 or LysoTracker in DRAM1 knockdown and control cell lines. (C) Percentage of colocalisation of Mm with LC3 in punctate and luminal patterns, in DRAM1 knockdown and control cell lines. (D) Percentage of colocalisation of LysoTracker with LC3-negative Mm and LC3-positive punctate and luminal Mm patterns, in DRAM1 knockdown and control cell lines. Bar graphs show the data from three independent experiments, where the mean of each replicate is indicated with a colored symbol. In total, 18 ROIs were analysed per control or DRAM1 knockdown (KD1-3) group, with the following total numbers of Mm observations per group: Ctrl = 527, KD1 = 551, KD2 = 516, KD3 = 537. Statistical significance was assessed by beta-binomial logistic regression with Dunnett’s multiple test correction. Error bars represent the standard deviation of the three independent replications of the experiment. (* p < 0.05; ** p < 0.01; *** p < 0.001).