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Figure 6
Enhanced insulin signaling observed in vdra-/- ;vdrb-/- zebrafish. (A) Quantitative real-time PCR showed the cyp24a1, cyp2r1 and cyp27b1 transcript levels in the liver of vdra-/;vdrb-/- fish and control siblings at 100 dpf; (B, C) Quantitative real-time PCR showed the insulin and insra, transcriptional levels in the liver of vdra-/;vdrb-/- fish and control siblings at 100 dpf; (D) AKT, p-AKT, S6, and p-S6 protein levels in liver samples of vdra-/-;vdrb-/- fish and control siblings at 100 dpf; (E) Quantification of relative p-AKT/AKT and p-S6/S6 protein levels from western blot analysis.
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Full text @ Front Endocrinol (Lausanne)