ZFIN Image: Yan et al., 2023, Figure 1

Image

Figure Caption

Figure 1

Generation of isl2a and isl2b knockout zebrafish utilizing CRISPR-Cas9 technology. (A) Amino acid identity between zebrafish and human ISL2 proteins was performed using UCSC Blat software. (B) The isl2a-CRISPR-target site (black bold words) was designed in exon3 of isl2a and the CRISPR/Cas9-induced insertion of 13 nucleotides (red words) was selected for further investigation. (C) In the case of isl2b, the CRISPR-target site (bold words) was designed in exon2, and the induced deletion of five nucleotides (red words) was selected for further investigation. Genotypes, including wild-type and homozygotes, were analyzed and shown in the bottom panel. Both genotypes were predicted to produce truncated proteins.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)