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Fig. 5
Figure 5. Respirasome subcomplex preserves the steady-state levels of holo-CIII and CIV Blue-native PAGE and immunoblotting analysis of the CIII and CIV (A) in HEK293T cells with sequence-specific KD of six subunits from each module and control HEK293T cells (Ctrl). DDM-solubilized protein was separated by 3.5%–16% blue-native PAGE. CII (SDHA) was used as an internal control. Quantitative analysis of CIII and CIV (B) was performed with three independent experiments and presented as the means ± SEM. ∗p < 0.05, ∗∗p < 0.01.
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