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Figure 2.
RT-PCR quantification of the osteogenic differentiation marker genes ALP (A), Col I (B) and BMP2 (C) in the control and MOR treatment groups. (D) Runx2, OPN and GAPDH protein levels were detected by Western blotting in MC3T3-E1 cells. NOR (10 and 20 μM) significantly increased OPN (E) and Runx2 (F) protein levels. *p< 0.05, **p< 0.01 vs. Ctl. n = 6.
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