ZFIN Image: Chu et al., 2023, FIGURE 1

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Figure Caption

FIGURE 1

(A) Design of the CRISPR/Cas9 target sites of the guide RNA (gRNA) in dnd1. The gRNA was designed in exon 2, and the target site of the gRNA is indicated in red, while the sequence highlighted in yellow is PAM. The RNA-recognition motif (RRM) is the conserved functional domain encoded by exon 3 of the dnd1 gene. (B) Microinjection schematic with the component and concentration of the working solution. (C) Mutation identified in each F0 $F_{0}$ generation by T7E1 using 2% agarose gel. (D) Mutation efficiency analyzed using PCR on the caudal fin’s DNA.

Acknowledgments

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