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Fig. 8
TPM2 variants did not affect swim function. (A) Automated tracking of the startle response in 6 dpf larvae. EthoVision imaging software (Noldus) tracked larvae for 3 seconds after a mechanical stimulus. Red lines show the locomotor path of representative larvae. Endpoints are marked by a square. (B–D) Box plots showing swim function. The startle response in larvae that expressed E41K, K49Del, R91G, and E122K was not significantly different from larvae that expressed the benign variant E273K. Swim distance, average escape velocity, and maximum velocity of larvae that expressed TPM2 variants were reported by imaging software and normalized to larvae that expressed wild-type TPM2. Significance was determined by 1-way ANOVA. n ≥ 25 larvae per treatment. Error bars, SEM.