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Figure 4 Tracking individual fish behavior. Tracks of swim paths of seven fish created using idTracker from video recordings (a) before, and (b) during presentation of a short, 4 s duration sound stimulus. Tracks begin at locations 1 s pre- and terminate 4 s post-sound onset. (c). Box plots and jittered scattergrams showing distances of fish (n = 6) from correct (target) side after training in response to the presentation of sounds within a single trial (4). Data were obtained from 1 s before (PRE) and during the second (POST2) and third (POST3) second post-stimulus. Shorter distances from target indicate better learning. On average, fish were closer to target during POST2 and started to wander away during POST3. ** indicate statistically significant and *** indicates highly significant difference between means (horizontal line within each box). One stationary fish trapped behind the divider was not tracked and another starting at the divider was not included in the analysis. Aerator and underwater speaker are on the right and feeder tube on the left. Perimeter shows glass wall reflection as the image was captured by a camera placed below the tank.