Fig. 1

Myd88 is required for hyperinflammation but dispensable for emergency myelopoiesis induced by S1WT. Recombinant S1WT (+) or vehicle (−) were injected in the hindbrain ventricle (HBV) of 2 dpf Myd88-deficient Tg(mpx:eGFP) (A) or wild type (B–D) larvae. Neutrophil (A) and macrophage (neutral red positive cells) (B) recruitment and number were analyzed at 6, 12 and 24 hpi by fluorescence (A) or brightfield (B) microscopy, the transcript levels of the indicated genes were analyzed at 12 hpi by RT-qPCR in larval head and tail (C), and caspase-1 activity was determined at 24 hpi using a fluorogenic substrate (D). Each dot represents one individual and the mean ± S.E.M. for each group is also shown. P values were calculated using one-way ANOVA and Tukey multiple range test. RT-qPCR data are depicted as a heat map in C with higher expression shown in darker color. ns, not significant, **p ≤ 0.01, ***p ≤ 0.001. auf, arbitrary units of fluorescence.