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FIG 3
Germfree status of larvae does not affect HuNoV replication. (A to D) HuNoV replication in germfree and conventional larvae with genotypes GII.2 (A), GII.3 (B), GII.6 (C), and GII.4 (D). Calculated inocula (3 nL per zebrafish larva) are 1.5 × 102 (A), 8.2 × 103 (B), 1.3 × 103 (C), and 1.2 × 104, 1.2 × 103, and 1.2 × 102 (D) viral RNA copies. For all graphs, the bars represent viral RNA levels/zebrafish larva, quantified by RT-qPCR. The mean values ± SEM are presented. Mann-Whitney tests were performed. The dotted line represents the LLOQ. ns, not significant.
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