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Fig. 3
Figure 3.Neuronal activation associated with spreading depolarization (SD) as revealed by immediate early gene c-fos expression. Coronal sections of the tectum treated with PBS as the vehicle (A) or KCl (B) showing the expression of the immediate early gene-c-fos protein (black). Scale: 500 µm. C and D: magnified images of A and B respectively. Scale: 50 µm. E: bar graphs with dot-whisker plots of the signal intensity measured by optical density (arbitrary unit) of c-fos protein expression in the tectum of the zebrafish treated with vehicle (n = 4), or KCl (n = 7). **P < 0.01.
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Full text @ J. Neurophysiol.