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Fig. 4
Sialic acid distribution in regenerating zebrafish heart identified by lectin histochemistry.
a Lectin histochemistry showing the distribution of MAA, recognizing α-(2,3)-linked sialic acid, b SNA-I, binding to α-(2,6)-linked sialic acid and c WGA, binding to sialic acid and GlcNAc, in ventricle tissue. d MAA, e SNA-I and f WGA relative quantification. The lectin binding quantifications highlighted a similar decrease at 2 dpci and a subsequent increase in the following timepoints. Cardiomyocytes were identified by GFP positivity. IA injured area. Data are shown as mean with SD (n = 4 animals per group). Scale bar = 100 μm. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
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