ZFIN Image: Geng et al., 2022, Fig. 4.

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Fig. 4.

Prenatal inhibition of Top2 induces social communication impairments and increased perseveration in mouse.

(A) Experimental design for prenatal ICRF-193 injection and subsequent experiments for the offspring. L, left; R, right. (B to D) ICRF-193 (I, n = 16) reduces the total duration (B), total number (C), and mean duration (D) of social investigation compared to vehicle control ethanol (E, n = 20). (E to G) ICRF-193 (n = 16) does not reduce the total duration (E), total number (F), and mean duration (G) of novel object investigation compared to ethanol (n = 19). (H and I) ICRF-193 (n = 16) does not change the percentage of time spent in the center zone (H) and velocity (I) of mice in the open-field assay compared to ethanol (n = 18). (J and K) ICRF-193 (n = 16) reduces the total number (J) but increases the mean peak frequency (K) of USV compared to ethanol (n = 20). (L and M) ICRF-193 (n = 16) reduces the total number of alterations (the number of times a mouse enters an alternative arm compared to the previous trial) (L) while increasing the number of trials before the first alteration (M) compared to ethanol (n = 20) during a T-maze assay that consisted of eight trials. (N) ICRF-193 (n = 16) increases the coefficient of variation in the hole-board assay compared to ethanol (n = 20). *P < 0.05, **P < 0.01, and ****P < 0.0001.

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