Figure 10

Impact of a sapS deletion on S. aureus SA564 ability to produce biofilms. (A) Cells of S. aureus SA564 (black), SA564 ΔsapS (blue), and SA564 ΔsapS::sapS (grey) were cultivated in BHI for 48 h at 37 °C in a humid atmosphere using 96-well microtiter plates. The crystal violet assay was used to evaluate the entire biofilm formation. The optical density (550 nm) and the production of biofilm are directly correlated. The presentation includes the means as well as the standard errors for a total of six different replicates. (B–D) Influence of SapS on the ability of S. aureus SA564 to build biofilms on medical devices. (B) Photographs of fragments of S. aureus-loaded peripheral venous catheters (PVC) taken on day 5 after the initial inoculation (6.3-fold magnification). These findings are indicative of three separate tests. An uninfected PVC fragment incubated for 5 days in sterile medium served as control. (C,D) Total biomass and CFU rates of detached biofilms were calculated by measuring the OD₆₀₀ of the TSB solutions (B) and plate counting (D). The values of each individual experiment are represented by symbols, along with the median value (horizontal line) (n = 8). *, p < 0.05; ns, not significant (Kruskal–Wallis test and Dunn’s post hoc test, only the changes between SA564 and mutants are displayed).