Figure 2

Figure 2

Zebrafish Cleavage period embryos display furrow-related dynamic hyperpolarization. (A–BB) Still-frame representative max-projection images from time-lapse videos (Supplementary Video S1–S3). 1–16 cell stages of Tg (ubi: ASAP1) zebrafish embryos were imaged from the animal pole position. (A–G) Representative Vm images from 1–2 cell stage fish embryo. (H–U) Representative Vm images from 2–8 cell stage fish embryo. (V–BB) Representative Vm images from an 8–16 cell stage fish embryo. Areas of bright green indicate hyperpolarization. Yellow boxes show regions of interest (ROIs) for measuring fluorescence intensity over time. The white arrow in (O) points to the P2 furrow signal. Signals appeared before cleavage furrows formed and then fluctuated as cytokinesis progressed. (CC) Adjusted fluorescence intensity, ∆F_Adj, of ROIs in panels (H–N). (DD) Adjusted fluorescence intensity, ∆F_Adj, of ROIs in panels (O–U). All lines in panels (CC,DD) represent the change in adjusted fluorescence intensity of ROIs for the designated cleavage furrows over time. CF (center furrow), a fertilized embryo’s initial division plane. P1, parental cell one. P2, parental cell two. DC1, daughter cell one. DC2, daughter cell two. DC3, daughter cell three. DC4, daughter cell four. Time (lower right corner), hours: minutes: seconds. Scale Bar= 250 µm.